diff --git a/chrome/content/zotero/standalone/standalone.js b/chrome/content/zotero/standalone/standalone.js
index cb9e4e7e5e..e6bc926427 100644
--- a/chrome/content/zotero/standalone/standalone.js
+++ b/chrome/content/zotero/standalone/standalone.js
@@ -557,6 +557,8 @@ const ZoteroStandalone = new function() {
 			}
 			this.updateMenuItemCheckmark('view-menuitem-split-vertically', reader.splitType === 'vertical');
 			this.updateMenuItemCheckmark('view-menuitem-split-horizontally', reader.splitType === 'horizontal');
+			this.updateMenuItemCheckmark('view-menuitem-use-dark-mode-for-content', Zotero.Prefs.get('reader.useDarkModeForContent'));
+			this.updateMenuItemEnabled('view-menuitem-use-dark-mode-for-content', window.matchMedia('(prefers-color-scheme: dark)').matches);
 		}
 	
 		// Layout mode
diff --git a/chrome/content/zotero/xpcom/reader.js b/chrome/content/zotero/xpcom/reader.js
index 7899fac8c6..ba93218a7f 100644
--- a/chrome/content/zotero/xpcom/reader.js
+++ b/chrome/content/zotero/xpcom/reader.js
@@ -215,6 +215,7 @@ class ReaderInstance {
 				...Zotero.Intl.getPrefixedStrings('pdfReader.')
 			},
 			showAnnotations: true,
+			useDarkModeForContent: Zotero.Prefs.get('reader.useDarkModeForContent'),
 			fontFamily: Zotero.Prefs.get('reader.ebookFontFamily'),
 			onOpenContextMenu: () => {
 				// Functions can only be passed over wrappedJSObject (we call back onClick for context menu items)
@@ -507,6 +508,7 @@ class ReaderInstance {
 		this._prefObserverIDs = [
 			Zotero.Prefs.registerObserver('fontSize', this._handleFontSizeChange),
 			Zotero.Prefs.registerObserver('tabs.title.reader', this._handleTabTitlePrefChange),
+			Zotero.Prefs.registerObserver('reader.useDarkModeForContent', this._handleContentDarkModeChange),
 			Zotero.Prefs.registerObserver('reader.ebookFontFamily', this._handleFontFamilyChange),
 		];
 
@@ -832,6 +834,10 @@ class ReaderInstance {
 		await this.updateTitle();
 	};
 
+	_handleContentDarkModeChange = () => {
+		this._internalReader.useDarkModeForContent(Zotero.Prefs.get('reader.useDarkModeForContent'));
+	};
+
 	_handleFontFamilyChange = () => {
 		this._internalReader.setFontFamily(Zotero.Prefs.get('reader.ebookFontFamily'));
 	};
diff --git a/chrome/content/zotero/zoteroPane.xhtml b/chrome/content/zotero/zoteroPane.xhtml
index c5dda80876..3a55d95407 100644
--- a/chrome/content/zotero/zoteroPane.xhtml
+++ b/chrome/content/zotero/zoteroPane.xhtml
@@ -515,7 +515,14 @@
 									oncommand="ZoteroStandalone.currentReader.toggleVerticalSplit()"
 							/>
 							<menuseparator class="menu-type-reader"/>
-
+							<menuitem
+									id="view-menuitem-use-dark-mode-for-content"
+									class="menu-type-reader"
+									type="checkbox"
+									label="&zotero.pdfReader.useDarkModeForContent;"
+									oncommand="Zotero.Prefs.set('reader.useDarkModeForContent', !Zotero.Prefs.get('reader.useDarkModeForContent'))"
+							/>
+							<menuseparator class="menu-type-reader"/>
 							<menu id="layout-menu" label="&layout.label;">
 								<menupopup oncommand="ZoteroStandalone.onViewMenuItemClick(event)">
 									<menuitem
diff --git a/chrome/locale/en-US/zotero/zotero.dtd b/chrome/locale/en-US/zotero/zotero.dtd
index a4f8bed0b1..411873cbc3 100644
--- a/chrome/locale/en-US/zotero/zotero.dtd
+++ b/chrome/locale/en-US/zotero/zotero.dtd
@@ -333,3 +333,4 @@
 <!ENTITY zotero.pdfReader.transferFromPDF					"Import Annotations…">
 <!ENTITY zotero.pdfReader.rotatePageLeft                    "Rotate Page Left">
 <!ENTITY zotero.pdfReader.rotatePageRight                   "Rotate Page Right">
+<!ENTITY zotero.pdfReader.useDarkModeForContent             "Use Dark Mode for Content">
diff --git a/defaults/preferences/zotero.js b/defaults/preferences/zotero.js
index 9a4e4c1a08..b721d1cb8f 100644
--- a/defaults/preferences/zotero.js
+++ b/defaults/preferences/zotero.js
@@ -219,6 +219,7 @@ pref("extensions.zotero.scaffold.eslint.enabled", true);
 pref("extensions.zotero.tabs.title.reader", "titleCreatorYear");
 
 // Reader
+pref("extensions.zotero.reader.useDarkModeForContent", true);
 pref("extensions.zotero.reader.ebookFontFamily", "Georgia, serif");
 
 // Set color scheme to auto by default
diff --git a/reader b/reader
index 4b0c629ee1..6a281b3975 160000
--- a/reader
+++ b/reader
@@ -1 +1 @@
-Subproject commit 4b0c629ee10df3978abd84b665954b16fb176aeb
+Subproject commit 6a281b397531825a5bd973f0e8260eeb1c62b44d